inovmedИнновационная медицина КубаниInnovative Medicine of Kuban2541-9897Scientific Research Institute – Ochapovsky Regional Clinical Hospital No. 110.35401/2541-9897-2025-10-2-96-102inovmed-1197Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзучение физико-химических и биологических свойств О-карбоксиметилхитозана, полученного из хитозана пчелиного подмораPhysicochemical and Biological Properties of O-carboxymethyl Chitosan Obtained From Chitosan of Dead Beeshttps://orcid.org/0009-0009-3371-4054КурбоноваФ. Н.KurbonovaF. N.

Курбонова Феруза Нуруллоевна - доктор философии, доцент кафедры медицинской химии.

200118, Бухара, ул. Гиждувана, 23

Feruza N. Kurbonova - PhD, Associate Professor at the Department of Medical Chemistry, Bukhara State Medical Institute.

G’ijduvon ko’chasi, 23, Bukhara, 200118

kurbonova_f86@mail.ruhttps://orcid.org/0000-0002-1687-682XИхтияроваГ. А.IxtiyarovaG. A.

Ихтиярова Гулнoра Акмаловна - д. хим. н., профессор.

Ташкент

Gulnora A. Ixtiyarova - Dr. Sci. (Chem.), Professor, Tashkent State Technical University.

Tashkent

https://orcid.org/0000-0002-5039-5384ГригорьянА. Ю.GrigoryanA. Yu.

Григорьян Арсен Юрьевич - д. м. н., доцент кафедры оперативной хирургии и топографической анатомии.

Курск

Arsen Yu. Grigoryan - Dr. Sci. (Med.), Associate Professor at the Department of Operative Surgery and Topographic Anatomy, Kursk State Medical University.

Kursk

https://orcid.org/0000-0003-4508-8336АвиловаО.AvilovaO.

Авилова Ольга - доктор философии, ассистент-профессор (медицинское образование).

Форт-Лодердейл

Olga Avilova - PhD, Assistant Professor of Medical Education, Nova Southeastern University.

Fort Lauderdale

https://orcid.org/0009-0004-7833-6986ХоджиевД. Ю.KhojievD. Ya.

Ходжиев Дилмурод Яхшиевич - доктор философии, доцент кафедры оперативной хирургии и топографической анатомии.

Термез

Dilmurod Y. Khojiev - PhD, Associate Professor at the Department of Operative Surgery and Topographic Anatomy, Termez Branch of the Tashkent Medical Academy.

Termez

https://orcid.org/0000-0001-5890-5636ХудойназароваГ. А.XudoynazarovaG. A.

Худойназарова Гулбахор Акиевна - к. хим. н., профессор кафедры химии и технологии нефти и газа.

Бухара

Gulbahor A. Xudoynazarova - Cand. Sci. (Chem.), Professor at the Department of Chemistry and Oil and Gas Technology, Bukhara State University.

Bukhara

Бухарский государственный медицинский институтУзбекистанBukhara State Medical InstituteUzbekistanТашкентский государственный технический университетУзбекистанTashkent State Technical UniversityUzbekistanКурский государственный медицинский университетРоссияKursk State Medical UniversityRussian FederationЮго-восточный университет НоваСоединённые Штаты АмерикиNova Southeastern UniversityUnited StatesТермезский филиал Ташкентской медицинской академииУзбекистанTermez Branch of the Tashkent Medical AcademyUzbekistanБухарский государственный университетУзбекистанBukhara State UniversityUzbekistan20253006202510296102Copyright © Курбонова Ф.Н., Ихтиярова Г.А., Григорьян А.Ю., Авилова О., Ходжиев Д.Ю., Худойназарова Г.А., 20252025Курбонова Ф.Н., Ихтиярова Г.А., Григорьян А.Ю., Авилова О., Ходжиев Д.Ю., Худойназарова Г.А.Kurbonova F.N., Ixtiyarova G.A., Grigoryan A.Y., Avilova O., Khojiev D.Y., Xudoynazarova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.innovmedkub.ru/jour/article/view/1197Цель

Цель: Синтез карбоксиметилхитозанов из местного сырья Apis Mellifera, изучение их физико-химических свойств, определение молекулярной массы и биологической активности и создание на их основе антибактериальных биоразлагаемых полимерных композиций.

Методы

Методы: Синтезирован карбоксиметилхитозан из подмора пчел, определена его молекулярная масса методом вискозиметрии, степень ацетилирования методом кодуктометрического титрования. Изучены его физико-химические свойства методами ИК-, ЯМР- и РСА-спектроскопии, полученные результаты сопоставлены с квантово-химическими расчетами. В работе представлены результаты гистологического анализа для изучения биологической активности карбоксиметилхитозана.

Результаты

Результаты: Впервые синтезирован О-карбоксиметилхитозан из хитозана Apis Mellifera и определены оптимальные условия: концентрация гидроксида натрия, соотношение алкилирующего агента, температура и продолжительность процесса, кинетические параметры.

Впервые приготовлены и испытаны на белых крысах мази для ожоговых ран на основе О-карбоксиметилхитозана Apis Mellifera. Определена концентрация гидроксида натрия 30%, необходимая температура для проведения реакции 650 оС, продолжительность процесса реакции полимерообразования 3 часа, соотношение хитозана к монохлоруксусной кислоте 1:1, а соотношение хитозана к алкилирующему агенту ИПС 1:50.

Заключение

Заключение: Мазь на основе карбоксиметилхитозана оказывает противомикробное действие, обладает фармакологической активностью в виде уникального антисептического свойства, препарат не нарушает физиологические функции кожи, не вызывает аллергического состояния организма, не вызывая в нем токсикологических проблем.

Objective

Objective: To synthesize carboxymethyl chitosans (CMCS) from local raw material Apis mellifera, study their physicochemical properties, determine molecular weight and biological activity, and make CMCS-based antibacterial biodegradable polymer compositions.

Methods

Methods: CMCS was synthesized from dead bees; its molecular weight was determined by viscometry, and the degree of acetylation was established by coductometric titration. Its physicochemical properties were studied using infrared, nuclear magnetic resonance, and X-ray spectroscopy, and the results obtained were compared with quantum chemical calculations. The study presents the results of histological analysis to investigate the biological activity of CMCS.

Results

Results: For the first time, O-CMCS was synthesized from Apis mellifera chitosan, and we determined its optimal conditions: sodium hydroxide concentration, alkylating agent ratio, process temperature and duration, and kinetic parameters.

For the first time, ointments for burn wounds based on Apis mellifera O-CMCS were prepared and tested on white rats.

The sodium hydroxide concentration was found to be 30%; the required temperature for the reaction was 650 °C; the polymer formation reaction process lasted 3 hours; the ratio of chitosan to monochloroacetic acid was 1:1, and the ratio of chitosan to isopropyl alcohol was 1:50.

Conclusions

Conclusions: The CMCS-based ointment has an antimicrobial effect and a unique antiseptic property. It does not disrupt the physiological functions of the skin or cause allergic reactions and toxicological problems.

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The authors declare that there are no conflicts of interest present.