inovmedИнновационная медицина КубаниInnovative Medicine of Kuban2541-9897Scientific Research Institute – Ochapovsky Regional Clinical Hospital No. 110.35401/2541-9897-2022-25-4-68-74inovmed-605Research ArticleОБЗОРЫREVIEWSМетоды определения давности тромбозаMethods of thrombus age determinationhttps://orcid.org/0000-0002-1786-6906БогданА. П.BogdanA. P.

Богдан Александр Петрович, к. м. н., доцент кафедры кардиохирургии и кардиологии ФПК и ППС

 350063, Краснодар, ул. М. Седина, 4 

Alexander P. Bogdan, Cand. Sci. (Med.), Assistant Professor, Department of Cardiac Surgery and Cardiology, Faculty of Advanced Training and Professional Retraining of Specialists

 4, M. Sedina str., Krasnodar, 350063 

albo903@yandex.ruhttps://orcid.org/0000-0003-3010-492XБабичеваО. В.BabichevaO. V.

Бабичева Ольга Васильевна, к. м. н., доцент кафедрыкардиохирургии и кардиологии ФПК и ППС; врач-кардиолог

 350063, Краснодар, ул. М. Седина, 4 

Olga V. Babicheva, Cand. Sci. (Med.), Assistant Professor, Department of Cardiac Surgery and Cardiology, Faculty of Advanced Training and Professional Retraining of Specialists; Cardiologist

 4, M. Sedina str., Krasnodar, 350063 

https://orcid.org/0000-0002-2626-9273ПереверзеваА. А.PereverzevaA. A.

Переверзева Ангелина Андреевна, врач-ординатор, кафедра кардиохирургии и кардиологии ФПК и ППС 

 350063, Краснодар, ул. М. Седина, 4 

 Angelina A. Pereverzeva, Resident doctor, Department of Cardiac Surgery and Cardiology, Faculty of Advanced Training and Professional Retraining of Specialists 

 4, M. Sedina str., Krasnodar, 350063 

https://orcid.org/0000-0001-6403-3299БарбухаттиК. О.BarbukhattiK. O.

Барбухатти Кирилл Олегович, д. м. н., заведующийкардиохирургическим отделением № 2; заведующий кафедрой кардиохирургии и кардиологии ФПК и ППС

 350063, Краснодар, ул. М. Седина, 4 

Kirill O. Barbukhatti, Dr. Sci. (Med.), Head of the Cardiac Surgery Department no. 2; Head of the Department of Cardiac Surgery and Cardiology, Faculty of Advanced Training and Professional Retraining of Specialists 

 4, M. Sedina str., Krasnodar, 350063 

Кубанский государственный медицинский университетРоссияKuban State Medical UniversityRussian FederationКубанский государственный медицинский университет; Научно-исследовательский институт – Краевая клиническая больница №1 им. проф. С.В. ОчаповскогоРоссияKuban State Medical University; Scientific Research Institute – Ochapovsky Regional Clinical Hospital no. 1 Russian FederationКубанский государственный медицинский университет; Научно-исследовательский институт – Краевая клиническая больница №1 им. проф. С.В. ОчаповскогоРоссияKuban State Medical University; Scientific Research Institute – Ochapovsky Regional Clinical Hospital no. 1Russian Federation202230122022046874Copyright © Богдан А.П., Бабичева О.В., Переверзева А.А., Барбухатти К.О., 20222022Богдан А.П., Бабичева О.В., Переверзева А.А., Барбухатти К.О.Bogdan A.P., Babicheva O.V., Pereverzeva A.A., Barbukhatti K.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.innovmedkub.ru/jour/article/view/605

Актуальность: Венозные тромбоэмболические осложнения – грозное состояние с высоким риском летального исхода, нередко встречается в практике врача любой специальности. Своевременная диагностика и грамотная тактика ведения пациентов с тромбоэмболическими осложнениями являются ключевыми в благоприятном исходе заболевания. Актуальными проблемами для науки являются поиск и разработка диагностических подходов с целью получения полной характеристики тромботического события. Определение давности тромбоза – это один из таких значимых критериев, четкое понимание которого позволит выбрать успешную стратегию лечения пациентов с подобными осложнениями.Цель исследования: На сегодняшний день оценка возраста тромба строится в основном на анамнестических данных больного, которые не всегда отражают истинную картину, а также визуализирующих методиках, опирающихся на косвенные признаки. Поэтому неэффективность терапии описываемых состояний в некоторых случаях можно объяснить недооценкой возраста тромба. Развитие научных исследований в этом направлении представляется перспективным и способным привести к улучшению результатов лечения пациентов, страдающих венозными тромбоэмболическими осложнениями. В представленной обзорной статье авторы проанализировали имеющиеся в литературе методы определения давности тромбоза.

Relevance: Venous thromboembolic complications are a formidable condition with a high mortality risk, that is rather common in the practice of a physician of any specialty. Therefore, timely diagnosis and correct management of patients with thromboembolic complications are key to a favorable outcome of the disease. An urgent problem for science nowadays is the search and development of diagnostic approaches that give a complete description of a thrombotic event. One of these characteristics is the determination of the age of thrombosis; it is a clear understanding of this criterion that makes it possible to choose a successful treatment strategy for patients with similar complications.Aim of study: To date, the assessment of the age of a thrombus is based mainly on the patient’s anamnestic data, which do not always correspond to the real situation, and imaging techniques based on indirect signs. Therefore, the ineffectiveness of therapy for the described conditions in some cases can be explained by an underestimation of the age of the thrombus. The development of scientific research in this direction seems promising and can lead to an improvement in the results of treatment of patients suffering from venous thromboembolic complications. This article is a review of the methods for thrombus age determination presented in the literature.

возраст тромбозавенозные тромбоэмболииультразвуковая эластографиятромболизисгистоструктура тромбаage of thrombusvenous thromboembolismultrasound elastographythrombolysisthrombus histostructureReferencesSilverstein MD, Heit JA, Mohr DN, et al. Trends in the incidence of deep vein thrombosis and pulmonary embolism: a 25-year population-based study. Arch Intern Med. 1998;158(6):585–593. PMID: 9521222. https://doi.org/10.1001/archinte.158.6.585Silverstein MD, Heit JA, Mohr DN, et al. Trends in the incidence of deep vein thrombosis and pulmonary embolism: a 25-year population-based study. Arch Intern Med. 1998;158(6):585–593. PMID: 9521222. https://doi.org/10.1001/archinte.158.6.585Spencer FA, Goldberg RJ, Lessard D, et al. Factors associated with adverse outcomes in outpatients presenting with pulmonary embolism: the Worcester Venous Thromboembolism Study. Circ Cardiovasc Qual Outcomes. 2010;3(4):390–394. PMID: 20606125. PMCID: PMC3248812. https://doi.org/10.1161/circoutcomes.110.937441Spencer FA, Goldberg RJ, Lessard D, et al. Factors associated with adverse outcomes in outpatients presenting with pulmonary embolism: the Worcester Venous Thromboembolism Study. Circ Cardiovasc Qual Outcomes. 2010;3(4):390–394. PMID: 20606125. PMCID: PMC3248812. https://doi.org/10.1161/circoutcomes.110.937441White RH, Zhou H, Murin S, et al. Effect of ethnicity and gender on the incidence of venous thromboembolism in a diverse population in California in 1996. Thromb Haemost. 2005;93(2):298–305. PMID: 15711746. https://doi.org/10.1160/th04-08-0506White RH, Zhou H, Murin S, et al. Effect of ethnicity and gender on the incidence of venous thromboembolism in a diverse population in California in 1996. Thromb Haemost. 2005;93(2):298–305. PMID: 15711746. https://doi.org/10.1160/th04-08-0506Beckman MG, Hooper WC, Critchley SE, et al. Venous thromboembolism: a public health concern. Am J Prev Med. 2010;38:S495–501. PMID: 20331949. https://doi.org/10.1016/j.amepre.2009.12.017Beckman MG, Hooper WC, Critchley SE, et al. Venous thromboembolism: a public health concern. Am J Prev Med. 2010;38:S495–501. PMID: 20331949. https://doi.org/10.1016/j.amepre.2009.12.017Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin. 2019;69(1):7–34. PMID: 30620402. https://doi.org/10.3322/caac.21551Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin. 2019;69(1):7–34. PMID: 30620402. https://doi.org/10.3322/caac.21551Dobesh PP. Economic burden of venous thromboembolism in hospitalized patients. Pharmacotherapy. 2009;29(8):943–953. PMID: 19637948. https://doi.org/10.1592/phco.29.8.943Dobesh PP. Economic burden of venous thromboembolism in hospitalized patients. Pharmacotherapy. 2009;29(8):943–953. PMID: 19637948. https://doi.org/10.1592/phco.29.8.943White RH. The epidemiology of venous thromboembolism. Circulation. 2003;107(23 Suppl 1):I4–8. PMID: 12814979. https://doi.org/10.1161/01.cir.0000078468.11849.66White RH. The epidemiology of venous thromboembolism. Circulation. 2003;107(23 Suppl 1):I4–8. PMID: 12814979. https://doi.org/10.1161/01.cir.0000078468.11849.66Nitta D, Mitani H, Ishimura R, et al. Deep vein thrombosis risk stratification. Int Heart J. 2013;54(3):166–170. PMID: 23774241. https://doi.org/10.1536/ihj.54.166Nitta D, Mitani H, Ishimura R, et al. Deep vein thrombosis risk stratification. Int Heart J. 2013;54(3):166–170. PMID: 23774241. https://doi.org/10.1536/ihj.54.166Spyropoulos AC, Hurley JS, Ciesla GN, et al. Management of acute proximal deep vein thrombosis: pharmacoeconomic evaluation of outpatient treatment with enoxaparin vs inpatient treatment with unfractionated heparin. Chest. 2002;122(1):108–114. PMID: 12114345. https://doi.org/10.1378/chest.122.1.108Spyropoulos AC, Hurley JS, Ciesla GN, et al. Management of acute proximal deep vein thrombosis: pharmacoeconomic evaluation of outpatient treatment with enoxaparin vs inpatient treatment with unfractionated heparin. Chest. 2002;122(1):108–114. PMID: 12114345. https://doi.org/10.1378/chest.122.1.108Monreal M, Agnelli G, Chuang LH, et al. Deep Vein Thrombosis in Europe-Health-Related Quality of Life and Mortality. Clin Appl Thromb Hemost. 2019;25:1076029619883946. PMID: 31840534. PMCID: PMC7027084. https://doi.org/10.1177/1076029619883946Monreal M, Agnelli G, Chuang LH, et al. Deep Vein Thrombosis in Europe-Health-Related Quality of Life and Mortality. Clin Appl Thromb Hemost. 2019;25:1076029619883946. PMID: 31840534. PMCID: PMC7027084. https://doi.org/10.1177/1076029619883946Yusof NNM, McCann A, Little PJ, et al. Non-invasive imaging techniques for the differentiation of acute and chronic thrombosis. Thromb Res. 2019;177:161–171. PMID: 30921535. https://doi.org/10.1016/j.thromres.2019.03.009Yusof NNM, McCann A, Little PJ, et al. Non-invasive imaging techniques for the differentiation of acute and chronic thrombosis. Thromb Res. 2019;177:161–171. PMID: 30921535. https://doi.org/10.1016/j.thromres.2019.03.009Hess S, Madsen PH, Basu S, et al. Potential role of FDG PET/CT imaging for assessing venous thromboembolic disorders. Clin Nucl Med. 2012;37(12):1170–1172. PMID: 23154475. https://doi.org/10.1097/rlu.0b013e318279bf73Hess S, Madsen PH, Basu S, et al. Potential role of FDG PET/CT imaging for assessing venous thromboembolic disorders. Clin Nucl Med. 2012;37(12):1170–1172. PMID: 23154475. https://doi.org/10.1097/rlu.0b013e318279bf73Wilbur J, Shian B. Diagnosis of deep venous thrombosis and pulmonary embolism. Am Fam Physician. 2012;86(10):913–919. PMID: 23157144.Wilbur J, Shian B. Diagnosis of deep venous thrombosis and pulmonary embolism. Am Fam Physician. 2012;86(10):913–919. PMID: 23157144.Wheeler HB, Anderson FA Jr. Diagnostic methods for deep vein thrombosis. Haemostasis. 1995;25(1–2):6–26. PMID: 7896224. https://doi.org/10.1159/000217140Wheeler HB, Anderson FA Jr. Diagnostic methods for deep vein thrombosis. Haemostasis. 1995;25(1–2):6–26. PMID: 7896224. https://doi.org/10.1159/000217140Aggarwal A, Bhalotra AR, Suresh V, et al. Assessing the age of deep vein thrombus: A need for future perioperative medicine and anesthesia. Anesth Essays Res. 2016;10(1):1–2. PMID: 26957680. PMCID: PMC4767089. https://doi.org/10.4103/0259-1162.167803Aggarwal A, Bhalotra AR, Suresh V, et al. Assessing the age of deep vein thrombus: A need for future perioperative medicine and anesthesia. Anesth Essays Res. 2016;10(1):1–2. PMID: 26957680. PMCID: PMC4767089. https://doi.org/10.4103/0259-1162.167803Screaton NJ, Gillard JH, Berman LH, et al. Duplicated superficial femoral veins: a source of error in the sonographic investigation of deep vein thrombosis. Radiology. 1998;206(2):397–401. PMID: 9457192. https://doi.org/10.1148/radiology.206.2.9457192Screaton NJ, Gillard JH, Berman LH, et al. Duplicated superficial femoral veins: a source of error in the sonographic investigation of deep vein thrombosis. Radiology. 1998;206(2):397–401. PMID: 9457192. https://doi.org/10.1148/radiology.206.2.9457192Cronan JJ. Venous thromboembolic disease: the role of US. Radiology. 1993;186(3):619–630. PMID: 8430164. https://doi.org/10.1148/radiology.186.3.8430164Cronan JJ. Venous thromboembolic disease: the role of US. Radiology. 1993;186(3):619–630. PMID: 8430164. https://doi.org/10.1148/radiology.186.3.8430164Schmitt C, Montagnon E, Hadj Henni A, et al. Shear Wave Induced Resonance Elastography of Venous Thrombi: A Proof-of-Concept. IEEE Transactions on Medical Imaging. 2013;32(3):565–577. PMID: 23232414. https://doi.org/10.1109/tmi.2012.2231093Schmitt C, Montagnon E, Hadj Henni A, et al. Shear Wave Induced Resonance Elastography of Venous Thrombi: A Proof-of-Concept. IEEE Transactions on Medical Imaging. 2013;32(3):565–577. PMID: 23232414. https://doi.org/10.1109/tmi.2012.2231093Yi X, Wei X, Wang Y, et al. Role of real-time elastography in assessing the stage of thrombus. Int Angiol. 2017;36(1):59–63. PMID: 27070908. https://doi.org/10.23736/s0392-9590.16.03610-5Yi X, Wei X, Wang Y, et al. Role of real-time elastography in assessing the stage of thrombus. Int Angiol. 2017;36(1):59–63. PMID: 27070908. https://doi.org/10.23736/s0392-9590.16.03610-5Mumoli N, Mastroiacovo D, Giorgi-Pierfranceschi M, et al. Ultrasound elastography is useful to distinguish acute and chronic deep vein thrombosis. J Thromb Haemost. 2018;16(12):2482–2491. PMID: 30225971. https://doi.org/10.1111/jth.14297Mumoli N, Mastroiacovo D, Giorgi-Pierfranceschi M, et al. Ultrasound elastography is useful to distinguish acute and chronic deep vein thrombosis. J Thromb Haemost. 2018;16(12):2482–2491. PMID: 30225971. https://doi.org/10.1111/jth.14297Aslan A, Barutca H, Ayaz E, et al. Is real-time elastography helpful to differentiate acute from subacute deep venous thrombosis? A preliminary study. J Clin Ultrasound. 2018;46(2):116–121. PMID: 28940429. https://doi.org/10.1002/jcu.22522Aslan A, Barutca H, Ayaz E, et al. Is real-time elastography helpful to differentiate acute from subacute deep venous thrombosis? A preliminary study. J Clin Ultrasound. 2018;46(2):116–121. PMID: 28940429. https://doi.org/10.1002/jcu.22522Ненарочнов С.В., Новикова Я.В., Куликов В.Г. и др. Метод оценки эмболоопастности венозных тромбов с использованием соноэластографии. Фундаментальные исследования. 2011;10(2):329–332.Nenarochnov SV, Novikova YaV, Kulikov VG, et al. The Evaluation of the Venous Thromboembolic Complication Risk Using Elastography. Fundamental Research. 2011;10(2):329–332. (In Russ.).Paluch Ł, Nawrocka-Laskus E, Dąbrowska A, et al. Ultrasound Elastography for Determination of the Age of Venous Thrombi. Evaluation of Thrombus Evolution in Patients After Sclerotherapy. Pol J Radiol. 2017;82:88–91. PMID: 28289480. PMCID: PMC5321172. https://doi.org/10.12659/pjr.899517Paluch Ł, Nawrocka-Laskus E, Dąbrowska A, et al. Ultrasound Elastography for Determination of the Age of Venous Thrombi. Evaluation of Thrombus Evolution in Patients After Sclerotherapy. Pol J Radiol. 2017;82:88–91. PMID: 28289480. PMCID: PMC5321172. https://doi.org/10.12659/pjr.899517Fang J, Chen CK, Peng JY, et al. Changes in backscattered ultrasonic envelope statistics as a function of thrombus age: an in vitro study. Ultrasound Med Biol. 2015;41(2):498–508. PMID: 25542488. https://doi.org/10.1016/j.ultrasmedbio.2014.09.024Fang J, Chen CK, Peng JY, et al. Changes in backscattered ultrasonic envelope statistics as a function of thrombus age: an in vitro study. Ultrasound Med Biol. 2015;41(2):498–508. PMID: 25542488. https://doi.org/10.1016/j.ultrasmedbio.2014.09.024Гольдина И.М. Новые подходы к ультразвуковой диагностике эмбологенного венозного венозного тромбоза. Журнал им. Н.В. Склифосовского «Неотложная медицинская помощь». 2013;(4):20–25.Goldina IM. New Ideas to Ultrasound diagnostics of Embologenic Venous Thrombosis. Russian Sklifosovsky Journal “Emergency Medical Care”. 2013;(4):20–25. (In Russ.).Drescher R, Freesmeyer M. PET angiography: Application of early dynamic PET/CT to the evaluation of arteries. AJR Am J Roentgenol. 2013;201(4):908–911. PMID: 24059383. https://doi.org/10.2214/ajr.12.10438Drescher R, Freesmeyer M. PET angiography: Application of early dynamic PET/CT to the evaluation of arteries. AJR Am J Roentgenol. 2013;201(4):908–911. PMID: 24059383. https://doi.org/10.2214/ajr.12.10438Nazaroğlu H, Ozmen CA, Akay HO, et al. 64-MDCT pulmonary angiography and CT venography in the diagnosis of thromboembolic disease. AJR Am J Roentgenol. 2009;192(3):654–661. PMID: 19234261. https://doi.org/10.2214/ajr.07.3939Nazaroğlu H, Ozmen CA, Akay HO, et al. 64-MDCT pulmonary angiography and CT venography in the diagnosis of thromboembolic disease. AJR Am J Roentgenol. 2009;192(3):654–661. PMID: 19234261. https://doi.org/10.2214/ajr.07.3939van Dam LF, Dronkers CEA, Gautam G, et al. Detection of upper extremity deep vein thrombosis by magnetic resonance noncontrast thrombus imaging. J Thromb Haemost. 2021;19(8):1973–1980. PMID: 34018662. PMCID: PMC8361740. https://doi.org/10.1111/jth.15394van Dam LF, Dronkers CEA, Gautam G, et al. Detection of upper extremity deep vein thrombosis by magnetic resonance noncontrast thrombus imaging. J Thromb Haemost. 2021;19(8):1973–1980. PMID: 34018662. PMCID: PMC8361740. https://doi.org/10.1111/jth.15394Вихрова Н.Б., Долгушин Б.И., Панов В.О. и др. Лучевые методы диагностики в определении структуры опухолевого тромбоза в нижней полой вене при раке почки. Онкоурология. 2015;11(3):40–45. http://doi.org/10.17650/1726-9776-2015-11-3-40-45Vikhrova NB, Dolgushin BI, Panov VO, et al. Radiodiagnostic methods in determining the structure of tumor thrombus in the inferior vena cava in kidney cancer. Cancerurology. 2015;11(3):40–45. (In Russ.). http://doi.org/10.17650/1726-9776-2015-11-3-40-45Saha P, Humphries J, Modarai B, et al. Leukocytes and the natural history of deep vein thrombosis: current concepts and future directions. Smith Arterioscler Thromb Vasc Biol. 2011;31(3):506–112. PMID: 21325673. PMCID: PMC3079895. https://doi.org/10.1161/atvbaha.110.213405Saha P, Humphries J, Modarai B, et al. Leukocytes and the natural history of deep vein thrombosis: current concepts and future directions. Smith Arterioscler Thromb Vasc Biol. 2011;31(3):506–112. PMID: 21325673. PMCID: PMC3079895. https://doi.org/10.1161/atvbaha.110.213405Andia ME, Saha P, Jenkins J, et al. Fibrin-targeted magnetic resonance imaging allows in vivo quantification of thrombus fibrin content and identifies thrombi amenable for thrombolysis. Arterioscler Thromb Vasc Biol. 2014;34(6):1193–1198. PMID: 24723557. PMCID: PMC4195984. https://doi.org/10.1161/atvbaha.113.302931Andia ME, Saha P, Jenkins J, et al. Fibrin-targeted magnetic resonance imaging allows in vivo quantification of thrombus fibrin content and identifies thrombi amenable for thrombolysis. Arterioscler Thromb Vasc Biol. 2014;34(6):1193–1198. PMID: 24723557. PMCID: PMC4195984. https://doi.org/10.1161/atvbaha.113.302931Corti R, Osende J, Fayad ZA, et al. In vivo noninvasive detection and age definition of arterial thrombus by MRI. J Am Coll Cardiol. 2002;39(8):1366–1373. PMID: 11955857. https://doi.org/10.1016/s0735-1097(02)01754-0Corti R, Osende J, Fayad ZA, et al. In vivo noninvasive detection and age definition of arterial thrombus by MRI. J Am Coll Cardiol. 2002;39(8):1366–1373. PMID: 11955857. https://doi.org/10.1016/s0735-1097(02)01754-0Drescher R, Freesmeyer M. PET angiography: Application of early dynamic PET/CT to the evaluation of arteries. AJR Am J Roentgenol. 2013;201(4):908–911. PMID: 24059383. https://doi.org/10.2214/ajr.12.10438Drescher R, Freesmeyer M. PET angiography: Application of early dynamic PET/CT to the evaluation of arteries. AJR Am J Roentgenol. 2013;201(4):908–911. PMID: 24059383. https://doi.org/10.2214/ajr.12.10438Rondina MT, Lam UT, Pendleton RC, et al. (18)F-FDG PET in the evaluation of acuity of deep vein thrombosis. Clin Nucl Med. 2012;37(12):1139–1145. PMID: 23154470. PMCID: PMC3564643. https://doi.org/10.1097/rlu.0b013e3182638934Rondina MT, Lam UT, Pendleton RC, et al. (18)F-FDG PET in the evaluation of acuity of deep vein thrombosis. Clin Nucl Med. 2012;37(12):1139–1145. PMID: 23154470. PMCID: PMC3564643. https://doi.org/10.1097/rlu.0b013e3182638934Housmand S, Salavati A, Hess S, et al. The role of molecular imaging in diagnosis of deep vein thrombosis. Am J Nucl Med Mol Imaging. 2014;4(5):406–25. PMID: 25143860. PMCID: PMC4138136.Housmand S, Salavati A, Hess S, et al. The role of molecular imaging in diagnosis of deep vein thrombosis. Am J Nucl Med Mol Imaging. 2014;4(5):406–25. PMID: 25143860. PMCID: PMC4138136.Taillefer R. Radiolabeled peptides in the detection of deep venous thrombosis. Semin Nucl Med. 2001;31(2):102–123. PMID: 11330782. https://doi.org/10.1053/snuc.2001.21268Taillefer R. Radiolabeled peptides in the detection of deep venous thrombosis. Semin Nucl Med. 2001;31(2):102–123. PMID: 11330782. https://doi.org/10.1053/snuc.2001.21268Березовский Д.П., Грибенников С.Н., Додохова М.А. Тромбозы в судебной медицине. Медицинский вестник Северного Кавказа. 2011;1(21):79–82.Berezovsky DP, Gribennikov SN, Dodokhova MA. Thrombosis in forensic medicine. Medical news of North Caucasus. 2011;1(21):79–82. (In Russ.).Митрофанова Л.Б., Аминева Х.К. Макроскопический и органометрический анализ сердца в патологии: пособие для врачей под ред. Ковальского Г.Б. Санкт-Петербург: ГПАБ; 1998:60.Mitrofanova LB, Amineva KhK. Macroscopic and organometric analysis of the heart in pathology: guidelines for physicians. Kovalsky GB (ed). Saint Petersburg: GPAB; 1998:60. (In Russ.).Fineschi V, Turillazzi E, Neri M, et al. Histological age determination of venous thrombosis: a neglected forensic task in fatal pulmonary thrombo-embolism. Forensic Sci Int. 2009;186(1–3):22–28. PMID: 19203853. https://doi.org/10.1016/j.forsciint.2009.01.006Fineschi V, Turillazzi E, Neri M, et al. Histological age determination of venous thrombosis: a neglected forensic task in fatal pulmonary thrombo-embolism. Forensic Sci Int. 2009;186(1–3):22–28. PMID: 19203853. https://doi.org/10.1016/j.forsciint.2009.01.006Fuijkschot WW, Groothuizen WE, Appelman Y, et al. Inflammatory cell content of coronary thrombi is dependent on thrombus age in patients with ST-elevation myocardial infarction. J Cardiol. 2017;69(1):394–400. PMID: 27836374. https://doi.org/10.1016/j.jjcc.2016.10.003Fuijkschot WW, Groothuizen WE, Appelman Y, et al. Inflammatory cell content of coronary thrombi is dependent on thrombus age in patients with ST-elevation myocardial infarction. J Cardiol. 2017;69(1):394–400. PMID: 27836374. https://doi.org/10.1016/j.jjcc.2016.10.003Furukoji E, Gi T, Yamashita A, et al. CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study. Thromb J. 2016;14:46. PMID: 27895532. PMCID: PMC5120412. https://doi.org/10.1186/s12959-016-0122-0Furukoji E, Gi T, Yamashita A, et al. CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study. Thromb J. 2016;14:46. PMID: 27895532. PMCID: PMC5120412. https://doi.org/10.1186/s12959-016-0122-0Nosaka M, Ishida Y, Kuninaka Y, et al. Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med. 2012;126(3):421–425. PMID: 22361951. https://doi.org/10.1007/s00414-012-0680-zNosaka M, Ishida Y, Kuninaka Y, et al. Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med. 2012;126(3):421–425. PMID: 22361951. https://doi.org/10.1007/s00414-012-0680-zNosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic macrophage-derived cytokines and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med. 2013;127(5):937–942. https://doi.org/10.1007/s00414-013-0873-0Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic macrophage-derived cytokines and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med. 2013;127(5):937–942. https://doi.org/10.1007/s00414-013-0873-0Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation. Int J Legal Med. 2015;129(5):1021–1025. PMID: 25616628. https://doi.org/10.1007/s00414-015-1147-9Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation. Int J Legal Med. 2015;129(5):1021–1025. PMID: 25616628. https://doi.org/10.1007/s00414-015-1147-9Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic fibrocytes and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med. 2017;131(1):179–183. PMID: 27757576. https://doi.org/10.1007/s00414-016-1465-6Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of intrathrombotic fibrocytes and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med. 2017;131(1):179–183. PMID: 27757576. https://doi.org/10.1007/s00414-016-1465-6Nosaka M, Ishida Y, Kimura A, et al. Detection of intrathrombotic endothelial progenitor cells and its application to thrombus age estimation in a murine deep vein thrombosis model. Int J Legal Med. 2017;131(6):1633–1638. PMID: 28828624. https://doi.org/10.1007/s00414-017-1668-5Nosaka M, Ishida Y, Kimura A, et al. Detection of intrathrombotic endothelial progenitor cells and its application to thrombus age estimation in a murine deep vein thrombosis model. Int J Legal Med. 2017;131(6):1633–1638. PMID: 28828624. https://doi.org/10.1007/s00414-017-1668-5Nosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of MMP-2 and MMP-9 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med. 2010;124(5):439–444. PMID: 20623133. https://doi.org/10.1007/s00414-010-0484-yNosaka M, Ishida Y, Kimura A, et al. Immunohistochemical detection of MMP-2 and MMP-9 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med. 2010;124(5):439–444. PMID: 20623133. https://doi.org/10.1007/s00414-010-0484-yNosaka M, Ishida Y, Kimura A, et al. Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasisinduced deep vein thrombosis model and its application to thrombus age determination. Int J Legal Med. 2009;123(3):235–240. PMID: 19194719. https://doi.org/10.1007/s00414-009-0324-0Nosaka M, Ishida Y, Kimura A, et al. Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasisinduced deep vein thrombosis model and its application to thrombus age determination. Int J Legal Med. 2009;123(3):235–240. PMID: 19194719. https://doi.org/10.1007/s00414-009-0324-0Stein-Merlob AF, Kessinger CW, Erdem SS, et al. Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study. Theranostics. 2015;5(12):1317–1327. PMID: 26516370. PMCID: PMC4615735. https://doi.org/10.7150/thno.12494Stein-Merlob AF, Kessinger CW, Erdem SS, et al. Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus AgeDependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study. Theranostics. 2015;5(12):1317–1327. PMID: 26516370. PMCID: PMC4615735. https://doi.org/10.7150/thno.12494Maffeis V, Nicolè L, Rago C, et al. Histological criteria for age determination of fatal venous thromboembolism. Int J Legal Med. 2018;132(3):775–780. PMID: 29052040. https://doi.org/10.1007/s00414-017-1705-4Maffeis V, Nicolè L, Rago C, et al. Histological criteria for age determination of fatal venous thromboembolism. Int J Legal Med. 2018;132(3):775–780. PMID: 29052040. https://doi.org/10.1007/s00414-017-1705-4

The authors declare that there are no conflicts of interest present.