inovmedИнновационная медицина КубаниInnovative Medicine of Kuban2541-9897Scientific Research Institute – Ochapovsky Regional Clinical Hospital No. 110.35401/2541-9897-2024-9-4-44-53inovmed-958Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИллюстрация распределения последовательностей ДНК и их частот в гене MEP2 у десяти видов CandidaIllustration of the Distribution of DNA Sequences and Their Frequencies Within the MEP2 Gene Across Ten Candida Specieshttps://orcid.org/0000-0001-8988-5957ДавудА. А.DawoodA. A.

Давуд Али Адел - PhD, ассистент-профессор, заместитель заведующего кафедрой анатомии, медицинский колледж.

41002, Мосул, ул. Аль-Маджмуа

Ali A. Dawood - PhD, Assistant Professor, Deputy Head of the Department of Anatomy, College of Medicine, University of Mosul.

Al Majmoaa St, Mosul, 41002

aad@uomosul.edu.iqУниверситет МосулаИракUniversity of MosulIraq202430122024044453Copyright © Давуд А.А., 20242024Давуд А.А.Dawood A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.innovmedkub.ru/jour/article/view/958

Виды грибов рода Candida влияют на здоровье кожи человека и периодически могут приводить к таким смертельным заболеваниям, как рак кожи. Источник азота в питательной среде регулирует экспрессию гена MEP2. Белок MEP2 может регулировать количество аммония, доступного клеткам, напрямую влияя на ассимиляцию аммония. Снижение экспрессии MEP2 устраняет его потенциал индуцировать филаментацию.

Целью данной работы было изучение серийной визуализации гена MEP2 с помощью подходов CGR и FCGR. Кроме того, было исследовано влияние мутаций в штаммах C. albicans на докинг MEP2 и оксида лаурамина (LDAO). Ген MEP2 был выбран для десяти видов Candida из банка данных NCBI, чтобы сравнить последовательности ДНК с использованием традиционных методов и методов представления (CGR и FCGR). Молекулярный докинг между MEP2 и LDAO был осуществлен с помощью сервера HDOCK.

По результатам CGR, C. margitis, C. orthopsilosis, C. dubliniensis, C. theae и C. albicans имеют примерно 65% одинаковых характеристик. По результатам FCGR, между C. albicans, C. theae, C. dubliniensis, C. orthopsilosis и C. Margitis наблюдается 75% сходства. В некоторых штаммах, но не во всех, мутация в консервативной области белка вызывала изменение в стыковочном остатке LDAO с MEP2.

Протоколы CGR и FCGR считаются практичными и надежными инструментами для определения сходства белков и последовательностей ДНК. Примерно 80% существующих алгоритмов определения множественных выравниваний последовательностей аналогичны традиционным методам. Стоит отметить, что таргетированное лечение возможно, поскольку определение мутаций MEP2 имеет решающее значение при использовании Candida в качестве источника азота.

Candida species have an impact on human skin health and occasionally can lead to catastrophic conditions, such as skin cancer. The nitrogen supply in growth media regulates MEP2 gene expression. MEP2 protein may regulate the amount of ammonium accessible to cells by directly affecting ammonium assimilation. Reducing MEP2 expression removed its potential to drive filamentous growth.

This study aimed to examine serial imaging of the MEP2 gene with chaos game representation (CGR) and frequency chaos game representation (FCGR). In addition, the effect of mutations in Candida albicans strains on MEP2 docking with lauramine oxide (LDAO) was investigated. The MEP2 gene was selected for 10 Candida species from the National Center for Biotechnology Information to compare DNA sequences using conventional and portray methods (CGR and FCGR). The molecular docking between MEP2 and LDAO was determined using the HDOCK server.

CGR findings revealed that Candida margitis, Candida orthopsilosis, Candida dubliniensis, Candida theae, and C albicans had approximately 65% of the same characteristics. According to FCGR, there was a 75% similarity between C albicans, C theae, C dubliniensis, C orthopsilosis, and C margitis. In certain strains, but not all a mutation in the conserved region of the protein caused a change in the docking residue of LDAO with MEP2.

The CGR and FCGR protocols are considered practical and reliable tools for identifying protein and DNA sequence similarities. Approximately 80% of the existing algorithms for determining multiple sequence alignments are similar to traditional methods. Targeted treatment will be possible as determining MEP2 mutations is crucial for using Candida as a nitrogen source.

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The authors declare that there are no conflicts of interest present.