On the issue of obtaining platelet-rich plasma
https://doi.org/10.35401/2500-0268-2022-25-1-38-43
Abstract
Objective: To determine the optimal technological modes for the preparation of platelet-rich plasma (PRP) using standard laboratory equipment.
Material and methods: Blood for the research was taken from 25 healthy volunteers. Its centrifugation was performed on a standard CM-6M laboratory centrifuge using various modes and two types of vacuum tubes with lithium heparin containing separation gel and without it. The number of platelets and leukocytes was calculated in the upper, lower and middle layers of the obtained plasma sample.
Results: Plasma samples obtained during centrifugation modes from 415 to 1660 g for 10 minutes using test tubes that do not contain separation gel are optimal in terms of the number of platelets. Plasma intake from the lower layer of the obtained sample after centrifugation is always accompanied by the inclusion of leukocytes in its composition, which can lead to undesirable tissue reactions when it is used.
Conclusion: To obtain PRP, it is possible to use standard laboratory equipment in the centrifugation mode from 415 to 1660 g for 10 minutes using test tubes that do not contain separation gel. Plasma sampling for clinical use should be carried out from the middle layer of the obtained sample.
About the Authors
S. B. Bazlov
Kuban State Medical University
Russian Federation
Russian Federation
Sergey B. Bazlov, Cand. Sci. (Med.), Associate Professor, Department of Faculty and Hospital Surgery
4, M. Sedina str., Krasnodar, 350063
K. I. Melkonian
Kuban State Medical University
Russian Federation
Russian Federation
Karina I. Melkonian, Cand. Sci. (Med.), Associate Professor, Head of the Central Research Laboratory
Krasnodar
T. V. Rusinova
Kuban State Medical University
Russian Federation
Russian Federation
Tatiana V. Rusinova, Cand. Sci. (Bio.), Research Fellow, Central Research Laboratory
Krasnodar
K. I. Popandopulo
Kuban State Medical University
Russian Federation
Russian Federation
Konstantin I. Popandopulo, Dr. Sci. (Med.), Head of the Department of Faculty and Hospital Surgery
Krasnodar
N. V. Marchenko
Kuban State Medical University
Russian Federation
Russian Federation
Nikolay V. Marchenko, Cand. Sci. (Med.), Associate Professor, Department of Faculty and Hospital Surgery
Krasnodar
D. D. Shevchuk
Kuban State Medical University
Russian Federation
Russian Federation
Daniil D. Shevchuk, 5th year student, Medical Faculty
Krasnodar
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Review
For citations:
Bazlov S.B., Melkonian K.I., Rusinova T.V., Popandopulo K.I., Marchenko N.V., Shevchuk D.D. On the issue of obtaining platelet-rich plasma. Innovative Medicine of Kuban. 2022;(1):38-43. (In Russ.) https://doi.org/10.35401/2500-0268-2022-25-1-38-43
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