Enhanced liver regeneration when using cell therapy after liver resection

Abstract

Background. The need to find the most effective ways to restore liver function after performing its resection.

Objective. To evaluate the effectiveness of the use of stem cell technology in liver resection in an experiment.

Material and Methods. 210 C57 black mice were included in the experiment. All individuals were divided into 4 groups, 60 individuals in groups 1–3 and 30 individuals in group 4. Individuals of groups 1–3 underwent liver resections in the amount of 60–65% of the total liver mass. To the individuals of group 1, stem cells were administered intraoperatively intravenously, group 2 – intraoperatively intraportally. No stem cells were introduced to individuals of group 3. Individuals of group 4 (n = 30) were removed from the experiment to determine the average liver mass and obtain stem cells.

Results. On the 10th day after the operation, the liver mass of mice in group 2 corresponded to normal indicators, in group 1 it was 79% of the norm, in group 3 – 62.6% of the norm. The level of total protein and albumin on day 10 after surgery in group 2 was within normal limits, in group 1 – 75% and 87% of the norm, in group 3 – 51.7% and 64.5% of the norm respectively. On the 10th day after the operation, the AST level in group 2 was 4% higher than the baseline, in group 1 – 26%, in group 3 – 58% higher than the baseline. The level of AST and alkaline phosphatase on day 10 after surgery in group 1 was 51% and 189% higher than the initial level, in group 2 – 5.9% and 49%, in group 3 – 90% and 266% higher respectively.

Conclusion. The use of stem cell technology in liver resection contributes to a faster restoration of liver function in the postoperative period.